RNA

Part:BBa_K2259009:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)


SynORI framework RNA I - replication Inhibitor (Group D-NC)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequences was taken from wildtype ColE1 origin ignoring the RNA II segment for RNA I to be controlled independedly.


Source

Wildtype ColE1 sequence was taken from addgene, mutated and ordered from IDT.

References